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KMID : 0368420050480030263
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Journal of Plant Biology
2005 Volume.48 No. 3 p.263 ~ p.263
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Isolation and characterization of squalene synthase cDNA fromcentella asiatica (L) urban
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Kim Ok-Tae
Seong Nak-Sul
Kim Min-Young
Hwang Baik
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Abstract
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We have cloned and characterized a gene for squalene synthase (SQS) fromCentella asiatica (L) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence ofpSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern analysis revealed that one copy might exist in the C.asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels ofCaSQS mRNA.
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KEYWORD
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asiaticoside, Centella asiatica, squalene synthase, triterpene saponins
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